Aim This study sought to determine the role of free radicals derived from mitochondria in the vasculature in the recognized age‐related endothelial dysfunction of human skeletal muscle feed arteries (SMFAs). Methods A total of 44 SMFAs were studied with and without acute exposure to the mitochondria‐targeted antioxidant MitoQ and nitric oxide synthase (NOS) blockade. The relative abundance of proteins from the electron transport chain, phosphorylated (p‐) to endothelial (e) NOS ratio, manganese superoxide dismutase (MnSOD) and the mitochondria‐derived superoxide (O2−) levels were assessed in SMFA. Endothelium‐dependent and endothelium‐independent SMFA vasodilation was assessed in response to flow‐induced shear stress, acetylcholine (ACh) and sodium nitroprusside (SNP). Results MitoQ restored endothelium‐dependent vasodilation in the old to that of the young when stimulated by both flow (young: 68 ± 5; old: 25 ± 7; old + MitoQ 65 ± 9%) and ACh (young: 97 ± 4; old: 59 ± 10; old + MitoQ: 98 ± 5%), but did not alter the initially uncompromised, endothelium‐independent vasodilation (SNP). Compared to the young, MitoQ in the old diminished the initially elevated mitochondria‐derived O2− levels and appeared to attenuate the breakdown of MnSOD. Furthermore, MitoQ increased the ratio of p‐eNOS to NOS and the restoration of endothelium‐dependent vasodilation in the old by MitoQ was ablated by NOS blockade. Conclusion This study demonstrated that MitoQ reverses age‐related vascular dysfunction by what appears to be an NO‐dependent mechanism in human SMFAs. These findings suggest that mitochondria‐targeted antioxidants may have utility in terms of counteracting the attenuated blood flow and vascular dysfunction associated with advancing age.