Objective Previous studies have found that psychological safety is central to effective team functioning and teamwork. Research has also found that psychological safety is dependent on work locus of control (WLOC). Specifically, external WLOC (i.e., perceived lack of personal control over work life) is negatively associated with psychological safety. However, there is limited understanding of underlying mechanisms, such as trust, which may affect this relationship. Method Surveys from 131 adult employees from Western Australia were collected from four different organisations. Results Results indicated a negative relationship between participants’ expression of external WLOC and psychological safety. Results also indicated that trust significantly moderated the relationship between WLOC and psychological safety. Conclusion These findings are valuable for all organisations that wish to increase psychological safety among team members for enhanced productivity and employee wellbeing.