The competing endogenous circular RNA ADAMTS14 suppressed hepatocellular carcinoma progression through regulating microRNA‐572/regulator of calcineurin 1
Journal of Cellular Physiology
Published online on October 14, 2018
Abstract
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- "\nAbstract\nEmerging evidence have discovered that circular RNAs (circRNAs) may
serve as diagnostic or tumor promising biomarkers. This study aimed to investigate
how circular RNA ADAMTS14 (circADAMTS14) regulates microRNA‐572/\nregulator of calcineurin
1(miR‐572/\nRCAN1) in hepatocellular carcinoma (HCC). The expression profiles of
circRNA/microRNA (mRNA) between HCC tissues and paired adjacent tissues were analyzed
via microarray analysis. The expressions of circADAMTS14, miR‐572, and \nRCAN1 were
measured by real‐time polymerase chain reaction (PCR). The protein expression level
of \nRCAN1 in HCC cells was detected by western blot. The viability and apoptosis
levels of HCC cell lines were measured by the cell counting Kit‐8 (CCK‐8) assay
and fluorescence‐activated cell sorter. The invasiveness and migration of cells
were detected based on the transwell and wound‐healing assay, respectively. The
dual‐luciferase reporter assays were used to reveal circADAMTS14 and \nRCAN1 as
a potential target of miR‐572, which was predicted by TargetScan and miRBase. The
effect of circADAMTS14 on HCC cells was demonstrated by tumor formation in nude
mice in vivo. CircADAMTS14 and \nRCAN1 were lowly expressed in HCC clinical specimens
and cell lines using microarrays and qRT‐PCR, but miR‐572 inversely. Our study further
verified the direct interaction between circADAMTS14 and \nRCAN1 with miR‐572 via
the dual‐luciferase reporter gene assay. Overexpressed circADAMTS14 and \nRCAN1
induced apoptosis of HCC cells and inhibited cell proliferation and invasion. But
overexpressed miR‐572 could decrease apoptosis of HCC cells and promote proliferation
and invasion. In vivo, circADAMTS14 inhibited the tumor growth, correlated positively
with the protein expression levels of \nRCAN1. Our results demonstrated that circADAMTS14
might suppress HCC progression through regulating miR‐572/\nRCAN1 as the competing
endogenous RNA."
- Journal of Cellular Physiology, EarlyView.