Induction of apoptotic but not autophagic cell death by Cinnamomum cassia extracts on human oral cancer cells
Journal of Cellular Physiology
Published online on October 14, 2018
Abstract
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- "\nAbstract\nCinnamomum cassia has been widely studied in different fields to reveal
its antidiabetic, antidepressive, antiviral, anti‐inflammatory, antiosteoporotic,
and anticancer effects. Its antimalignant activities have been explored in lung
cancer, breast cancer, colorectal cancer, and even oral cancer, but the detailed
signaling mechanism and effects of this plant on animal models need to be clarified.
In the current study, \nC. cassia extract (CCE) was used to investigate the antitumorigenesis
mechanism in vitro and in vivo. The major constituents of CCE used in this study
were coumarin, cinnamic acid, and cinnamic aldehyde. CCE reduced the viability,
number, and colony formation of human oral cancer cells, and induced their apoptosis.
Caspase‐3 activation, Bcl‐2 reduction, and phosphatidylserine inversion were involved
in CCE‐stimulated apoptosis. CCE also enhanced the expression of autophagic markers,
including acidic vesicular organelle, microtubule‐associated protein 1 light chain
3‐I, autophagy‐related protein 14, rubicon, and p62. The combined treatment of CCE
and caspase inhibitor significantly restored mitochondrial membrane potential (Δ\nψ\nm)
and cell viability. However, the combined treatment of CCE and autophagy inhibitor
further reduced the cell viability indicating that autophagy might be a survival
pathway of CCE‐treated SASVO3 cells. In contrast, CCE treatment for 12 days did
not adversely affect SASVO3 tumor‐bearing nude mice. CCE also elicited dose‐dependent
effects on the decrease in tumor volume, tumor weight, and Ki‐67 expression. These
results suggested that CCE showed the potential for the complementary treatment
of oral caner."
- Journal of Cellular Physiology, EarlyView.