--- - "\n\n\n\n\n\nAbstract\nChlorantraniliprole is an anthranilic diamide insecticide that binds to the insect ryanodine receptor (RyR) and induces an uncontrolled release of Ca2+, resulting in paralysis and ultimately death of the target insects. Recently, it was reported that chlorantraniliprole‐resistant diamondback moths, \nPlutella xylostella Linnaeus, have mutations in their RyR. In this study, we developed two different chlorantraniliprole‐resistant \nDrosophila melanogaster strain. The resistance ratio (RR) of the low‐concentration chlorantraniliprole‐treated resistant (Low‐Res) strain was 2.3, while that of the high‐concentration chlorantraniliprole‐treated resistant (High‐Res) strain was 21.3. The LC\n50 of the untreated control (Con) strain was 23.8~25.9 ppm, which was significantly higher than that reported for the susceptible diamondback moth (0.03~0.51 ppm). The high LC\n50 of the Con may be because the helix S2 amino acid sequence of \nD. melanogaster RyR (\nDmRyR) is identical to the I4790M mutation of the chlorantraniliprole‐resistant diamondback moths, resulting in a lower binding affinity of \nDmRyR for chlorantraniliprole. Among the tested detoxification enzymes, the activity of esterase was significantly increased in the two Res strains, but glutathione \nS‐transferases and acetylcholinesterase were significantly decreased in the two Res strains. The cross‐resistance of the High‐Res strain to other insecticides with different modes of actions (MoAs) revealed that the RRs of the neuronal acetylcholine receptor allosteric and competitive modulators were significantly increased, while those of the Na\n2+ channel modulators were significantly reduced. Our studies showed that RRs against the same insecticide vary with the treatment concentration, and that RRs against other insecticides with different MoAs can be altered." - 'Archives of Insect Biochemistry and Physiology, Volume 99, Issue 4, December 2018. '