--- - |2- Biplot of the representative two canonical dimensions within strains and CHCs of Anastrepha ludens males. Small circles indicate variability of the data, crossed circles (centroids) represent the mean for each strain. C = compound. Abstract In this study cuticular hydrocarbons (CHCs) were characterized from wings of individual unmated males of different Anastrepha ludens (Loew) mass‐reared strains of different ages (3 and 19‐day‐old): (a) a standard mass‐reared colony (control), (b) a genetic sexing strain, (c) a selected strain, (d) a hybrid strain, and (e) wild males. We found that the hydrocarbon profiles in all males included two n‐alkanes, five monomethyl alkanes, and two alkenes. CHCs ranged from C26 to C31. The most prominent peaks were 2‐methyloctacosane (2‐Me‐C28), n‐nonacosene (C29:1), 2‐methyltriacontane (2‐Me‐C30), and n‐hentriacontene (C31:1). Significant variations in the CHC amounts of the mass‐reared strains were observed from Day 9 and thereafter. Comparison of CHCs using multivariate and canonical analyses across ages and among mass‐reared strains and wild males revealed qualitative and quantitative differences. The relative amounts of C29:1 and 2‐Me‐C30 were significantly higher across age groups in the mass‐reared strains than those in the wild males. In contrast, amounts of n‐nonacosane (C29) significantly increased in wild males as they aged. Through statistical analyses, we inferred that CHC amounts vary with age. Wild males differed significantly from the mass‐reared strains in the amount of C29, and the genetic sexing strain Tap‐7 had significantly higher values for 2‐methylhexacosane (2‐Me‐C26). In contrast the selected and control strain differed from the other strains in amounts of C29:1 and 2‐Me‐C30. We suggest that differential profiles in hydrocarbon composition among the strains may be mainly due to environmental pressures. - 'Archives of Insect Biochemistry and Physiology, Volume 99, Issue 4, December 2018. '