Effects of nebulized N‐acetylcystein on the expression of HMGB1 and RAGE in rats with hyperoxia‐induced lung injury
Journal of Cellular Physiology
Published online on November 27, 2018
Abstract
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- "\nHigh mobility group box 1 (HMGB1) and receptor for advanced glycation end product
(RAGE) were involved in the pathogenesis of hyperoxia‐induced lung injury, inhalation
of N‐acetylcysteine might alleviate hyperoxia‐induced lung injury by regulating
the expression of HMGB1 and RAGE. \n\n\n\n\n\n\nAbstract\n\nObjective\nTo investigate
the role of high mobility group box 1 (HMGB1) and receptor for advanced glycation
end product (RAGE) in the lungs of hyperoxia‐induced rats and the effect of N‐acetlycystein
(NAC).\n\n\nMethods\nA model of hyperoxic lung injury was established, rats in the
NAC intervention, and control, hyperoxia group were given nebulized NAC aerosol,
nebulized same volume of saline once a day for 7 consecutive days, respectively.
Wet/dry (\nW/\nD) ratio of the lungs was determined to evaluate the edema of the
lung tissues. Conventional hematoxylin–eosin (HE) staining was used to observe the
pathological changes of lung tissues. Immunohistochemical staining was used to investigate
the expression of HMGB1 and RAGE in the lung tissues. Quantitative reverse‐transcription
polymerase chain reaction and western blot analysis were used to measured the changes
in the messenger RNA (mRNA) and protein expression of HMGB1 and RAGE, respectively.\n\n\nResults\nWeight
gain of the rats in the hyperoxia group was significantly slower than that in the
control group and intervention group (p < 0.05). HE staining results showed lung
tissues in the hyperoxia group were severely damaged compared with control group.
W/D ratio in hyperoxia group was significantly higher than that in control group
and intervention group (p < 0.05). Protein and mRNA expression of HMGB1 and RAGE
in the hyperoxia group were significantly higher than control group and intervention
group (p < 0.05).\n\n\nConclusion\nHMGB1 and RAGE were involved in the pathogenesis
of hyperoxia‐induced lung injury, inhalation of NAC might alleviate hyperoxia‐induced
lung injury by regulating the expression of HMGB1 and RAGE.\n"
- 'Journal of Cellular Physiology, EarlyView. '