Cholinergic activity regulates the secretome of epicardial adipose tissue: Association with atrial fibrillation
Journal of Cellular Physiology
Published online on November 27, 2018
Abstract
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- "\n\n\nMuscarinic receptor type 3 is upregulated after adipogenesis.\nEpicardial
fat from atrium or ventricles express this receptor and contain cholinergic activity.\nEpicardial
fat‐released proteins after cholinergic activity as possible predictors of postoperative
AF\n\n\n\n\n\nAbstract\nBotulinum toxin injection on epicardial fat, which inhibits
acetylcholine (ACh) release, reduced the presence of atrial fibrillation (AF) in
patients after heart surgery. Thus, we wanted to study the profile of the released
proteins of epicardial adipose tissue (EAT) under cholinergic activity (ACh treatment)
and their value as AF predictors. Biopsies, explants, or primary cultures were obtained
from the EAT of 85 patients that underwent open heart surgery. The quantification
of muscarinic receptors (mAChR) by real‐time polymerase chain reaction or western
blot showed their expression in EAT. Moreover, mAChR Type 3 was upregulated after
adipogenesis induction (p < 0.05). Cholinergic fibers in EAT were detected by
vesicular ACh transporter levels and/or acetylcholinesterase activity. ACh treatment
modified the released proteins by EAT, which were identified by nano‐high‐performance
liquid chromatography and TripleTOF analysis. These differentially released proteins
were involved in cell structure, inflammation, or detoxification. After testing
the plasma levels of alpha‐defensin 3 (inflammation‐involved protein) of patients
who underwent open heart surgery (\nn = 24), we observed differential levels between
the patients who developed or did not develop postsurgery AF (1.58 ± 1.61 ng/ml
vs. 6.2 ± 5.6 ng/ml; \np < 0.005). The cholinergic activity on EAT might suggest
a new mechanism for studying the interplay among EAT, autonomic nervous system dysfunction,
and AF."
- 'Journal of Cellular Physiology, EarlyView. '