["Acta Physiologica, Volume 231, Issue 2, February 2021. ", "\nAbstract\n\nAim\nThe pathogenesis of obesity has been associated with high intake of dietary fat, and some recent studies have explored the cellular mechanisms of oro‐sensory detection of dietary fatty acids. We further assessed the role of transient receptor potential canonical (TRPC) channels in oro‐sensory perception of dietary lipids.\n\n\nMethods\nWe determined by RT‐qPCR and western blotting the expression of TRPC3/6/7 channels in mouse fungiform taste bud cells (mTBC). Immunocytochemistry was used to explore whether TRPC3 channels were co‐expressed with fatty acid receptors. We employed wild‐type (WT) mTBC, and those transfected with small interfering RNAs (siRNAs) against TRPC3 or STIM1. Ca2+ signalling was studied in TBC from TRPC3−/− mice and their WT littermates.\n\n\nResults\nWe demonstrate that mouse fungiform taste bud cells (mTBC) express TRPC3, but not TRPC6 or TRPC7 channels, and their inactivation by siRNA or experiments on TBC from TRPC3−/− mice brought about a decrease in fatty acid‐induced gustatory Ca2+ signalling, coupled with taste bud CD36 lipid sensor. TRPC3 channel activation was found to be under the control of STIM1 in lingual mTBC. Behavioural studies showed that spontaneous preference for a dietary long‐chain fatty acid was abolished in TRPC3−/− mice, and in mice wherein lingual TRPC3 expression was silenced by employing siRNA.\n\n\nConclusion\nWe report that lingual TRPC3 channels are critically involved in fat taste perception.\n\n"]