In this study, transverse nonlinear vibration and instability analysis of a viscous-fluid-conveyed single-layered graphene sheet (SLGS) subjected to thermal gradient are investigated. The small-size effects on bulk viscosity and slip boundary conditions of nanoflow through Knudsen number (Kn), as a small size parameter is considered. Viscopasternak model is considered to simulate the interaction between the graphene sheet and the surrounding elastic medium. Continuum orthotropic plate model and relations of classical plate theory are used. The nonlocal theory of Eringen is employed to incorporate the small-scale effect into the governing equations of the graphene sheet. Differential quadrature method is employed to solve the governing differential equations for simply supported edges. The convergence of the procedure is shown and the effects of flow velocity, temperature change and aspect ratio on the frequency of the single-layered graphene sheet are investigated. Moreover, the critical flow velocities and the instability characteristic are determined. It is evident from the results that the natural frequency of nanosheet increases with rising temperature.