This article reports the result of an investigation into the effects of surface modulus, residual surface tension and bioliquid density on the vibration frequency of bioliquid-filled microtubule. Results show that influences of surface modulus and residual surface tension on the vibration frequency of bioliquid-filled microtubule are different. The influence of surface effect on the vibration frequency of bioliquid-filled microtubule is dependent on the vibration mode of microtubule and the bioliquid density in the microtubule.