In order to improve levitation capability and stability of ultrasonic standing wave, a novel levitation device was presented, which adopted concave spherical surface on the emitter and the reflector. Using ANSYS software, the acoustic field generated by the concave spherical emitting surface was analyzed and the formation of ultrasonic standing wave was simulated. Based on the simulation result, the distribution and maximum acoustic pressure under different radius of concave spherical surface on the emitter and the reflector were ascertained. Through the MATLAB simulation, the optimal structural parameter and levitation position were predicted. Based on the optimization result, the prototype of standing wave levitation device was designed and manufactured. In the laboratory, the radiation force was tested and levitation experiments were also carried out and the actual levitation position was in accordance with the simulation results. When the distance between the emitter and the reflector equaled to about 34.9 mm, three steel balls of 3 mm diameter could be levitated at the same time in three disparate nodes position, the levitation capability and stability were demonstrated to be enhanced largely.