Previous research has demonstrated that parents and children often have conflicting mate preferences. The present research was conducted among 443 Japanese university students. Using an existing scale designed to uncover parent‐offspring conflict over mate choice, the results revealed that children perceived having a potential partner with traits connoting poor genetic quality as being more unacceptable to themselves, and having a potential partner with traits connoting low parental investment and cooperation with the ingroup as being more unacceptable to their parent. A number of sex differences emerged. The highest potential for parent‐offspring conflict existed between female offspring and their father, and female offspring also rated traits connoting low social status as being more unacceptable to their parents, particularly to the father.