The purpose of this comparative study is to investigate the role of gender and culture in entrepreneurial orientation (EO) among students in the selected nations. EO dimensions are important variables in the study of organizational performance. We adopted Lumpkin and Dess’ EO dimensions. We statistically analyzed the collected data from 389 university students in the US (96), Korea (114), Fiji (80), and Malaysia (99). The results showed significant differences in most EO dimensions between genders and among the nations. Thus, it is important that customized approaches based on gender and unique cultural context are needed for developing EO among college students.