1.Recurring insulin‐induced hypoglycemia (RIIH) often occurs during the therapeutic management of insulin dependent diabetes mellitus. Controversy currently exists in the literature as to the ability of insulin and/or hypoglycemia to promote hypertension. Could insulin and/or hypoglycemia promote adverse pressor effects; if so, under what conditions and through what mechanism? Thus, the current study was performed to evaluate the hypothesis that recurrent insulin‐induced hypoglycemia produces hypertension via an induction in HO‐1 promoting a significant increase in endogenous CO. 2.Male Sprague Dawley rats were treated for 2 weeks with varying doses of subcutaneous insulin injections (1, 3, 5, 7, and 9 U/kg body weight) or vehicle and fed normal rat chow or zinc diet (1mM) for 2 weeks. Tail‐cuff blood pressure, food/water intake and blood glucose states were monitored daily. 3.A dose dependent decrease in blood glucose was observed. The blood pressure was significantly elevated in rats treated with 9 U/Kg and 7U/Kg doses as compared to other units. However, there was no change in urine output among all the groups. High zinc diet + 7U/Kg and HO‐1 inhibitor ZnDPBG (20mg/kg) treatments produced a significant reduction in blood pressure as compared to 7U/Kg alone. In addition, HO‐1 protein levels in heart and kidney and endogenous CO levels were reduced in high zinc diet + 7U/Kg vs 7U/Kg rats. 4.These results demonstrate that RIIH promotes hypertension and restoration of normal CO levels with a high zinc diet and ZnDPBG reduces the blood pressure. This article is protected by copyright. All rights reserved.