The present research sought to determine whether young female chess players would demonstrate stereotype threat susceptibility in a naturalistic environment. Data from 12 scholastic chess tournaments indicated that females performed worse than expected when playing against a male opponent, achieving 83% of the expected success based on their own and their opponent’s prerating. These effects were strongest for the youngest players in lower elementary school but also present for those in upper elementary. Stereotype threat susceptibility was most pronounced in contexts that could be considered challenging: when playing a strong or moderate opponent and when playing someone in a higher or the same grade. As evidence of disengagement, those most vulnerable to stereotype threat were less likely to continue playing in future chess tournaments. These results were not found in a matched comparison male group suggesting the outcomes were unique to stereotype threat and not universal to young chess players.