Background: Water-perfused high-resolution manometry (HRM) catheters with 36 unidirectional pressure channels have recently been developed but normal values are not yet available. Furthermore, the technique has not been validated and compared with solid-state HRM. We therefore aimed to develop normal values for water-perfused HRM and to assess the level of agreement between water-perfused HRM and solid-state HRM. Methods: We included 50 healthy volunteers (Mean age: 35 years, range: 21 - 64 years, 15 female). Water-perfused HRM and solid-state HRM were performed in a randomized order. Normal values were calculated as 5th and 95th percentile ranges and agreement between the two systems was assessed using intra-class correlations coefficient (ICC) statistics. Results: The 5-95th percentile range for contractile front velocity (CFV) was 3.0 - 6.6 cm s-1, for distal contractile integral (DCI) 141.6 - 3674 mmHg*s*cm, for distal contraction latency (DL) 6.2 - 8.7 s and for integrated relaxation pressure (IRP4s) 1.0 - 18.8 mmHg. Mean (SD) and ICC for water-perfused HRM and solid-state HRM were for CFV 4.4 (1.1) vs 3.9 (0.9) cm s-1, ICC: 0.49, for DCI 1189 (1023) vs 1092 (1019) mmHg*s*cm, ICC: 0.90, for DL 7.4 (0.8) vs 6.9 (0.9) s, ICC: 0.50 and for IRP4s 8.1 (4.8) vs 7.9 (5.1), ICC:0.39. Conclusions: The normal values for this water-perfused HRM system are only slightly different from previously published values with solid state HRM and moderate to good agreement was observed between the two systems with only small differences in outcome measures.