VPA uniquely induced hemorrhagic liposis of the cervical muscles in the chicken embryo model (CEM). Vitamin E (Vit E) and resveratrol (RV) have been cited to exhibit prominent antioxidative and glutathione (GSH)‐protecting effects. We hypothesized that Vit E and RV could ameliorate such kind pathological change. 120 day‐zero fertilized eggs were divided into 10 groups, 12 in each. VPA (60 μM), RV (at 0.2 and 2.0 μM) and Vit E (at 0.2 and 2.0 μM) in several combinations were tested with HH stage‐10 (day 1.5) CEM according to the method as indicated.RV and Vit E at 2.0 μM effectively rescued NTD in early stage CEM and inhibited the malformation rate (8.4 and 5.0%, respectively vs. the control 36.5±3.0%) (p<0.05), suppressing the serum homocysteine and S‐adenosylhomocysteine levels, downregulating the cervical muscular carnitine, triglycerides, H2O2, MDA, IL‐6 and ACC (p<0.05) and upregulating CPT1 (p<0.05) and glutathione (p<0.05). The hemorrhagic liposis of cervical muscles can be alleviated by RV and Vit E. Suggestively, the main action mechanism of RV and Vit E to rescue such VPA‐induced teratogenicity is through suppressing ROS and regenerating GSH. This article is protected by copyright. All rights reserved.