The aim of this study is to investigate the relationship of inflammation, myocardial fibrosis, and cardiac remodeling in patients with mild aortic stenosis (AS) as assessed by biomarkers and echocardiography. We consecutively evaluated 32 patients with mild AS and thirty age and gender matched, healthy individuals with normal aortic valves were recruited as control subjects for this study. Baseline echocardiography showed that left ventricular mass index (LVMI, 111.3 ± 26.9 g/m2 vs. 94.5 ± 18.2 g/m2, p‐value = 0.006) and left atrial volume index (LAVI, 27.5 ± 9.0 mm3/mm2 vs. ± 5.2 mm3/mm2, p‐value = 0.005) were significantly higher in patients with mild AS. Left atrial enlargement (LAVI >33 mm3/mm2, 32.4% vs. 3.3%, p‐value = 0.003) and elevated left ventricular filling pressure (E/e’ >15, 50.0% vs. 23.3%, p‐value=0.036) were higher in patients with mild AS. In the patients with mild AS, stepwise, multivariate linear regression analysis showed that the left ventricular end diastolic volume index (LVEDVI) was independently associated with matrix metalloproteinase (MMP)‐1(β= 0.371, p‐value=0.015), aortic valve mean pressure gradient (AVMPG) was independently associated with MMP‐2 (β= 0.19, p‐value=0.019), transforming growth factor (TGF)‐β (β=0.95, p‐value<0.001) and interleukin (IL)‐1(β= 0.17, p‐value=0.019) were independently associated with MMP‐2, and IL‐1 was independently associated with tissue inhibitor of matrix metalloproteinase (TIMP)‐1 (β= 0.68, p‐value=0.001). Myocardial fibrosis in mild AS is independently associated with three factors: LV volume overload, aortic valve pressure overload, and inflammation. This article is protected by copyright. All rights reserved.