Introduction. Pancreatic polypeptide (PP) is an anorexigenic hormone released from pancreatic F-cells upon food intake. We aimed to determine the effect of PP on gastric accommodation and gastric emptying in conscious Wistar HAN rats to investigate whether effects on motor function could contribute to its anorexigenic effects. Methods. Intragastric pressure (IGP) was measured through a chronically implanted gastric fistula during the infusion of a nutrient meal (Nutridrink; 0.5 ml min^-1). Rats were treated with PP (0, 33 and 100 pmol kg^-1 min^-1) in combination with L-NG-arginine methyl ester (L-NAME; 180 mg kg^-1 h^-1), atropine (3 mg kg^-1 h^-1) or vehicle. Furthermore, the effect of PP was tested after subdiaphragmal vagotomy of the stomach. Gastric emptying of a non-caloric and a caloric meal after treatment with 100 pmol kg^-1 min^-1 PP or vehicle was compared using X-rays. Results. PP significantly increased IGP during nutrient infusion compared to vehicle (p<0.01). L-NAME and atropine significantly increased IGP during nutrient infusion as compared to vehicle treatment (p<0.005 and <0.01 respectively). The effect of PP on IGP during nutrient infusion was abolished in the presence of L-NAME and in the presence of atropine. In vagotomized rats, PP increased IGP compared to intact controls (p<0.05). PP significantly delayed gastric emptying of both a non-caloric (p<0.05) and a caloric meal (p<0.005). Conclusion. PP inhibits gastric accommodation and delays gastric emptying, probably through inhibition of nitric oxide release. These results indicate that, besides the well-known centrally-mediated effects, PP might decrease food intake through peripheral mechanisms.