Aim To study the effect of Salvianolic acid B (Sal B) on lipopolysaccharide (LPS)‐induced disseminated intravascular coagulation (DIC) in rabbits. Methods Continuous infusion of LPS induced DIC model in rabbits. The treatments with Salvianolic acid B were started simultaneously with LPS infusion through the contralateral marginal ear vein. activated partial thromboplastin time (APTT), prothrombin time (PT), platelet count and fibrinogen concentration were measured; the plasma levels of fibrin‐fibrinogen degradation products (FDP) were detected; alanine aminotransferase (ALT) and blood urine nitrogen (BUN), the activity of protein C, antithrombinIII (ATIII) and the concentration of TNF‐α were determined. Results The gradual impairment of hemostatic parameters was induced by continuous infusion of LPS. The APTT, PT, BUN, ALT and plasma TNF‐α increased obviously, however, the platelet count, fibrinogen, FDP, protein C and ATIII decreased clearly. The intravenous administration of Salvianolic acid B attenuated the increased levels of APTT, PT, BUN, ALT and plasma TNF‐α, and the decreased levels of fibrinogen, platelet, FDP, protein C and ATIII induced by LPS infusion. Conclusion Salvianolic acid B has the effect against LPS induced DIC in rabbits This article is protected by copyright. All rights reserved.