The purpose of this study was to clarify the association of eNOS 894 G/T and ACE I/D genetic polymorphisms with risk of coronary heart disease (CHD) and to explore the effects of these polymorphisms on the therapeutic efficacy of salvianolate injection in Chinese CHD patients. 153 CHD patients and 198 healthy controls were enrolled in this study. We collected 5ml peripheral blood to extract DNA. Genetic diagnosis of eNOS 894G/T was determined by direct sequencing. Polymerase chain reaction‐restriction fragment length polymorphism analysis (PCR‐RFLP) was used to detect ACE I/D genotypes. We observed significant differences in the frequency distribution of eNOS and ACE polymorphisms between CHD patients and healthy controls (p<0.05). Binary logistic regression stepwise analysis revealed that the genotypes had an additive and dominant effect on patients’ therapeutic response (p<0.05). These data suggested that the genetic polymorphisms of ACE I/D and eNOS 894G/T probably play a role in the development of CHD and these genetic polymorphisms might affect the response to salvianolate injection in Chinese CHD patients. This article is protected by copyright. All rights reserved.