This study addresses the question how the setting of assessment influences maternal playing behavior with their 3-month-old infants across cultures. Mother–infant interactions of 338 dyads from two cultural communities (German middle-class and rural Cameroonian Nso) were videotaped either in their home or in a laboratory setting. Results indicate that both settings of assessment are appropriate to observe cultural differences in maternal interactional behavior. As expected, rural Nso mothers show more proximal interactional behavior than German middle-class mothers, who focus more on distal behavioral strategies. The laboratory setting amplifies cultural differences by culture-specific effects on the playing behavior. Whereas rural Nso mothers show increased activities in the lab, German middle-class mothers’ behavior seems to be inhibited.