This article explores the idea of agential multiplicity in medical treatment of childlessness. The analysis illustrates the kinds of agencies that emerge in the use of assisted reproductive technologies. The article begins with a discussion on feelings as participants in IVF treatment and as elements of women’s embodied experience. This is followed by an analysis of three consecutive steps of IVF: ovulation induction, assisted fertilization in the laboratory and embryo transfer. The article aims to show that feminist theory and praxis benefits from empirical analyses of lived bodily experiences as they take form in relation to non-human agencies. Also, it provides a view into how biological processes and material elements can be taken into account in anti-essentializing ways in feminist research.