The purpose of this study was to examine the factors influencing knowledge sharing in a public sector institution in Ghana. A case study of the factors influencing knowledge sharing among employees at Afigya Kwabre District Assembly in the Ashanti Region of Ghana was used as the research design. The purposive sampling technique was used to select the sample. A semi-structured interview guide was used for data collection. The data was analyzed using the thematic analysis technique. The study found mutual trust, respect and care for one another, the quest for organizational success, education and experience level of employees as the significant factors influencing knowledge sharing in the Afigya Kwabre District Assembly.