In his studies of culture, Juri Lotman implicitly expressed several ideas that have been rendered explicit by the contemporary mediasphere. The aim of the current article is to explicate a link between Lotmanian cultural semiotics and transmediality as one of today’s more innovative communicative practices. Transmediality is hereby located in the context of cultural autocommunication as a mechanism serving both creative and mnemonic functions. Thus, the notion is related not only to the questions of textual construction but, even more importantly, to text’s processual existence in culture in diverse media languages and discourses over time. By explaining the roots and developments of Lotman’s concept of autocommunicativity, which is central to his understanding of culture as a whole, the article simultaneously indicates the areas of his cultural semiotic studies that we consider relevant and fruitful for contemporary research into transmediality.