The utilization of mixed methods in prehospital research is relatively new. Its use may enhance research findings, but it is not without its challenges. This study used online databases to systemically search for literature relating to the application of mixed methods in prehospital research, in order to understand the place of mixed methods research in the prehospital setting. The prehospital field appears to be embracing mixed methods as an approach to research due to its ability to address health care questions in complex, diverse environments. However, supplemental literature in this area is limited, with mixed methods expertise being found in other health science areas. Researchers should endeavor to continue to contribute to this area through high-quality, rigorous mixed methods studies.