This study tests a comprehensive model linking Grey’s Anatomy viewing and perceived realism of this program with organ donation knowledge, barriers—including medical mistrust, disgust, bodily integrity, and superstition—and subsequent organ donation attitudes. In addition to testing the hypothesized structural model, ethnic differences were examined by way of (a) the multigroup method to test for differences in path coefficients, (b) multivariate analysis of variance to examine differences among the study variables, and (c) ² tests to assess differences in organ donation registrations among African Americans (n = 200), Caucasians (n = 200), and Latinos (n = 200). Support for the overall structural model was found and various differences emerged among the African American, Caucasian, and Latino sample across study variables. The results from this research are discussed with an emphasis on the theoretical and practical implications.