This article presents findings of the research by Nelen, Peters and Vanderhallen (2013b) regarding cross-border police cooperation in the Meuse-Rhine Euroregion. These findings are scrutinised in light of the conceptual framework of inter-organisational conflict (Scott, Austral Social Work 58:132–141, 2005) to provide an enhanced and more in-depth analysis of the possibilities and difficulties in international police cooperation. Potential conflict between cooperating organisations is identified by five levels of analysis: (i) inter-organisational; (ii) intra-organisational; (iii) inter-professional; (iv) interpersonal and (v) intra-personal. Obstacles for international police cooperation are mainly found at the inter-organisational level and interpersonal level. Particularly, the Euroregional police organisations and their case management systems are ill attuned, creating conflict in cooperation. The article concludes with the potential benefits for further police cooperation in the Meuse-Rhine Euroregion which are identified through the analysis and provides feedback on the conceptual framework.