The AMP-activated protein kinase (AMPK) is an endogenous energy sensor that regulates lipid and carbohydrate metabolism. Non-alcoholic fatty liver disease (NAFLD) is regarded as a hepatic manifestation of metabolic syndrome with impaired lipid and glucose metabolism, and increased oxidative stress. Our recent study showed that folic acid supplementation attenuated hepatic oxidative stress and lipid accumulation in high fat diet fed mice. The aim of the present study was to investigate the effect of folic acid on hepatic AMPK during high fat diet feeding and the mechanisms involved. Male C57BL/6J mice were fed a control diet (10% kcals fat), a high fat diet (60% kcals fat) or a high fat diet supplemented with folic acid (26mg/kg diet) for 5 weeks. Mice fed a high fat diet exhibited hyperglycemia, hepatic cholesterol accumulation and reduced hepatic AMPK phosphorylation. Folic acid supplementation restored AMPK phosphorylation (activation), and reduced blood glucose and hepatic cholesterol levels. Activation of AMPK by folic acid was mediated through an elevation of its allosteric activator AMP and activation of its upstream kinase, namely, liver kinase B1 (LKB1) in the liver. Consistent with in vivo findings, 5-methyltetrahydrofolate (bioactive form of folate) restored phosphorylation (activation) of both AMPK and LKB1 in palmitic acid-treated HepG2 cells. Activation of AMPK by folic acid might be responsible for AMPK-dependent phosphorylation of HMG-CoA reductase, leading to reduced hepatic cholesterol synthesis during high fat diet feeding. These results suggest that folic acid supplementation may improve cholesterol and glucose metabolism by restoration of AMPK activation in the liver.