This study presents a new scale to measure work values. The Work Values questionnaire (WVal) consists of 10 sets of 5 value items that respondents rank for importance. Each item expresses 1 of the 10 basic values of the Schwartz theory in the work context. Seven hundred fifty-nine employees from 94 organizations responded to the WVal. Confirmatory factor analysis (CFA) assessment of the 10 work values exhibited adequate factorial distinctiveness as well as high internal reliability. Multidimensional scaling analyses yielded a circular motivational structure of relations among the 10 work values similar to the higher order values in the basic values’ theory. We note and discuss meaningful differences in the importance of work values associated with gender, age, organizational tenure, and professional role. The study supports the applicability of the Schwartz basic value theory to the organizational setting, with some variations traceable to the work context. We discuss research possibilities and practical applications of the WVal.