One hundred nine people with autism spectrum disorder (ASD) completed the Depression Anxiety Stress Scales (DASS), and Stress Survey Schedule (SSS). Compared with Australian norms for DASS depression, anxiety, and stress, both males and females with ASD showed elevated scores. Females aged 25 to 44 years scored significantly higher on the DASS depression subscale than same aged males and younger males and females. Significant gender and age differences were evident on SSS subscales. In comparison with males, females were more stressed on Pleasant Events, Sensory/Personal Contact, and Social and Environmental subscales, and adult females in particular were more stressed on items around Change and Social Threats and Anticipation/Uncertainty. Young people were less stressed on seven of the eight SSS subscales than older people. It appears that emotional vulnerability in people with ASD varies according to gender and age, with adult females at particular risk.