Primary care physicians (PCPs) need a wide range of health information resources to manage their patients. The present study aimed to investigate the barriers that obstruct the health information seeking behavior of primary care physicians in Pakistan from their practice setting. A survey was carried out in 77 basic health units (BHUs), seven rural health centers (RHCs), and two tehsil headquarters (THQs) of Multan District, Pakistan. A three-part questionnaire containing participants’ demographic information, barriers in seeking health information, and required sources and services, was distributed to primary care physicians through post, email, and personal visits to participating healthcare facilities. The data was analyzed statistically using SPSS. Chi square and Kruskal Wallis tests were applied to determine if there were any significant differences in demographic information of different healthcare facilities participants, the barriers they faced in seeking health information, and the required sources and services available to them. The major barriers included: no onsite library, no health sciences librarian, scarcity of health information resources, inaccessibility of health information system, and lack of information and technological infrastructure. Consultation with seniors, improved connectivity and document delivery services were among the sources and services most required by primary care physicians. The study concluded that primary care physicians encounter multiple barriers that obstruct their health-related information seeking behavior, which compromises patient care. Medical librarians should design outreach library and information services in health sciences and health information literacy programs to overcome the barriers primary care physicians face while seeking health information from their practice settings.