Human trafficking is a growing crime in South Asia, particularly as economies move post-globalization. This mixed-method study explored several variables fueled by gender biases that create women’s vulnerability to human trafficking. Qualitative results supported the quantitative data from the World Development Indicator’s report that describe gross gender biases practiced in various South Asian countries. The most important findings reveal the current practices of gender bias, prostitution, and trafficking that are not recorded in the existing literature. A multi-dimensional regional practice model is proposed that could support female empowerment and international efforts to curb human trafficking in this region.