Despite the potential value of DNA evidence for criminal investigations and prosecution, we have a limited understanding of the way forensic evidence is used and its impact on case outcomes. This study uses qualitative data to describe the way investigators from the Houston Police Department use DNA evidence during investigations of sexual assaults. Results show DNA evidence has limited influence during investigations, and the value of DNA evidence is shaped by other evidentiary factors. The findings provide insight into the utility of DNA evidence, instances when DNA evidence is least and most useful, the importance of DNA evidence in comparison with other evidence, and the likely aggregate impact of DNA evidence across sexual assault cases.