Objective The spatial variation of dental caries in late Holocene southern South American populations will be analyzed using geostatistical methods. The existence of a continuous geographical pattern of dental caries variation will be tested. Methods The author recorded dental caries in 400 individuals, collated this information with published caries data from 666 additional individuals, and calculated a Caries Index. The caries spatial distribution was evaluated by means of 2D maps and scatterplots. Geostatistical analyses were performed by calculating Moran's I, correlograms and a Procrustes analysis. Results There is a relatively strong latitudinal continuous gradient of dental caries variation, especially in the extremes of the distribution. Moreover, the association between dental caries and geography was relatively high (m12 = 0.6). Although northern and southern samples had the highest and lowest frequencies of dental caries, respectively, the central ones had the largest variation and had lower rates of caries than expected. Conclusion The large variation in frequencies of dental caries in populations located in the center of the distribution could be explained by their subsistence strategies, characterized either by the consumption of wild cariogenic plants or cultigens (obtained locally or by exchange), a reliance on fishing, or the incorporation of plants rich in starch rather than carbohydrates. It is suggested that dental caries must be considered a multifactorial disease which results from the interaction of cultural practices and environmental factors. This can change how we understand subsistence strategies as well as how we interpret dental caries rates. Am. J. Hum. Biol., 2016. © 2016 Wiley Periodicals, Inc. Am. J. Hum. Biol. 28:825–836, 2016. © 2016Wiley Periodicals, Inc.