This study examines the relationship between prenatal testosterone exposure (PTE) and selection into entrepreneurship. We argue that the relationship between PTE and entrepreneurial intent is positive and mediated by general and domain‐specific risk‐taking related to financial investment and professional career. Using the second‐to‐fourth digit ratio (2D:4D) as noninvasive retrospective marker for PTE, we identify two‐step mediation effects of PTE on entrepreneurial intent through both general and domain‐specific risk‐taking. To account for possible socialization‐based effects, we control for gender and parental self‐employment. Applying ordinary least squares (OLS) regression analyses and structural equation models, we provide empirical evidence for a biological association between 2D:4D and entrepreneurial intent.