This study examined the factor structure and validity of the Acculturation Gap Conflicts Inventory (AGCI), a new instrument developed to measure the types of recurring conflicts that young people experience as part of the parent–child acculturation gap. Participants included 283 Hispanic young adults who completed the AGCI and existing measures of acculturation, family dynamics, psychosocial, and academic adjustment. Principal axis factor analysis revealed three factors with good internal consistency: Autonomy Conflicts, Conflicts over Preferred-Culture, and Dating/Being Out Late Conflicts. These factors correlated in the expected direction with acculturative stress and family dynamics variables. Autonomy Conflicts explained more than 25% of the variance in the acculturation gap conflicts items investigated, and this factor demonstrated incremental validity in predicting psychosocial and academic adjustment beyond the variance accounted for by other acculturative stress variables. The AGCI can be valuable to researchers from a variety of disciplines interested in measuring acculturation-related intergenerational conflicts among Hispanic youth that may be predictive of adjustment.