The scrutiny exerted by opposition parties in European Union (EU) affairs crucially contributes to the functioning of democratic accountability in the EU. While our knowledge about the extent of control activities has increased, we know little about their content. This article investigates the policy issues that opposition parties address in parliamentary questions about the EU. I argue that Eurosceptic opposition parties follow vote-maximizing strategies and selectively scrutinize very general aspects of the EU. My expectation is tested using a unique data collection of written questions in the Danish Folketing (1973–2013). I find evidence that the content of questions has been broadened over time and that Eurosceptic parties strongly emphasize general EU matters. The results point to the importance of political parties in making democratic accountability in the EU work.