It is well recognised in psychology that music has affective connotations and that musical stimuli can modify affective states. The aim of this study was to assess the affective connotations of 120 fifteen-second musical excerpts, covering both modern musical genres such as pop, rock, jazz, rap/R&B and electronic music (5 x N = 20), and classical music (N = 20). Expert judges used predetermined criteria to select excerpts with positive or negative valence that induced high arousal or low arousal. The excerpts were assessed by 50 undergraduate students (25 women) from different academic departments, aged between 18 and 28 years (M = 21.46 years, SD = 1.85). They listened to all 120 fragments and rated them with respect to six dimensions: valence, arousal, dominance, origin, subjective significance and imageability. Analyses showed that ratings were reliable, with high split-half correlations and Cronbach’s alpha estimates. We did not identify any gender differences concerning affective reactions to the music. Some music genre specificity was found for all measures, and initial music preference appeared to shape affective ratings. The results presented here will be of interest to researchers working on musical perception and the influence of music on affective outcomes and emotional regulation.