Aim This review critically evaluates the evidence on mechanosreceptors and pathways in the bladder urothelium that are involved in normal bladder filling signaling. Methods Evidence from in vitro and in vivo studies on 1) signaling pathways like the adenosine triphosphate pathway, cholinergic pathway and Nitrc Oxide and adrenergic pathway will be evaluated and 2) different urothelial receptors that are involved in bladder filling signaling like purinergic receptors, sodium channels and TRP channels will be evaluated. Other potential pathways and receptors will also be discussed. Results Bladder filling results in continuous changes in bladder wall stretch and exposure to urine. Both barrier and afferent signaling functions in the urothelium are constantly adapting to cope with these dynamics. Current evidence shows that the bladder mucosa host essential pathways and receptors that mediate bladder filling signaling. Intracellular calcium ion increase is a dominant factor in this signaling process. However, there is still no complete understanding how interacting receptors and pathways create a bladder filling signal. Currently, there are still novel receptors investigated that that could also be participating in bladder filling signaling. Conclusions Normal bladder filling sensation is dependent on multiple interacting mechanoreceptors and signaling pathways. Research efforts need to focus on how these pathways and receptors interact in order to fully understand normal bladder filling signaling. This article is protected by copyright. All rights reserved.