Deteriorated aerobic response to moderate exercise might precede the manifestation of clinical symptoms of non-communicable diseases. The purpose of the current study was to verify that the use of current wearable technologies for analysis of pulmonary oxygen uptake (VO₂) dynamics during a pseudorandom ternary sequence (PRTS) over-ground walking protocol is a suitable procedure for the investigation of the aerobic response in more realistic settings. A wearable accelerometer assessed the input changes delivered to the aerobic system. Eight adults (23.5±3.7 years old, 174±7 cm and 71.4±7.4 kg) performed two identical PRTS over-ground walking protocols. In addition, they performed on the cycle ergometer two identical pseudo-random binary sequence (PRBS) protocols and one incremental protocol for maximal VO₂ determination. In the frequency domain, mean normalized gain amplitude (MNG in %) quantified VO₂ dynamics. The MNG during PRTS was correlated (r=-0.80, p=0.01) with the VO₂ time constant obtain during cycling. The MNG estimated during PRBS was similar to the MNG estimated during PRTS (r=0.80, p=0.01). The maximal VO₂ correlated with the MNG obtained during the PRBS (r=0.79, p=0.01) and PRTS (r=0.78, p=0.02) protocols. In conclusion, PRTS over-ground walking protocol can be used to evaluate the aerobic system dynamics by the simultaneous measurement of VO₂ and hip acceleration. This study has shown that wearable technologies in combination with assessment of MNG, a novel indicator of system dynamics, open new possibilities to monitor cardiorespiratory health under conditions that better simulate activities of daily living than cardiopulmonary exercise testing performed in a medical environment.