This article presents the validation of the ‘Echelle de Motivation envers l’Activité Physique en contexte de Santé’ including the six forms of motivation underlined by self-determination theory. Study 1 underlines the content validity of a pool of 30 items (N = 20). Study 2 supports the six-factor structure validity of the 18-item Echelle de Motivation envers l’Activité Physique en contexte de Santé (N = 309). Study 3 (N = 191) confirms structure validity, as well as concurrent validity and 2-week temporal reliability. The Echelle de Motivation envers l’Activité Physique en contexte de Santé can be considered as a valid and reliable tool to use in prevention or rehabilitation contexts.