Allele rs2010963 C of the VEGFA gene is associated with the decreased risk of primary varicose veins in ethnic Russians

Shadrina, A. S., Smetanina, M. A., Sokolova, E. A., Shamovskaya, D. V., ianova, K. S., Shevela, A. I., Soldatsky, E. Y., Seliverstov, E. I., Demekhova, M. Y., Shonov, O. A., Ilyukhin, E. A., Voronina, E. N., Pikalov, I. V., Zolotukhin, I. A., Kirienko, A. I., Filipenko, M. L.

Phlebology: The Journal of Venous Disease

Published online on December 08, 2016

Abstract

Objective

To study the association of polymorphisms rs699947, rs2010963, rs3025039 in the VEGFA gene region and rs1870377, rs2305949, rs2071559 in the VEGFR2 gene region with the risk of primary varicose veins in ethnic Russians.

Methods

Genotypes were determined by real-time PCR allelic discrimination. The case group consisted of 448 patients with primary varicose veins and the control group comprised 609 individuals without a history of chronic venous disease. Association was studied by logistic regression analysis.

Results

Allele rs2010963 C was associated with the decreased risk of varicose veins (additive model of inheritance: odds ratio = 0.73, 95% confidence interval = 0.59–0.91, P = 0.004).

Conclusions

Our results provide evidence that polymorphism rs2010963 located in the 5' untranslated region of the VEGFA gene can influence genetic susceptibility to primary varicose veins in Russians. Otherwise, it can be in linkage disequilibrium with another functional single nucleotide polymorphism that can alter the level of vascular endothelial growth factor A protein.