Notch signaling is regulated by mechanical stimuli in various cell types. It has previously been reported that intermittent compressive stimuli enhanced sclerostin (SOST) expression in human periodontal ligament cells (hPDLs) by regulating transforming growth factor‐β (TGF‐β) expression. The aim of the present study was to determine the involvement of Notch signaling in the TGF‐β‐induced SOST expression in hPDLs. Cells were treated with intermittent compressive stress in a computer‐controlled apparatus for 24 h. The mRNA and protein expression of the cells were determined by real‐time polymerase chain reaction and Western blot analysis, respectively. In some experiments, the target signaling pathway was impeded by the addition of a TGF‐β receptor kinase inhibitor (SB431542) or a γ‐secretase inhibitor (DAPT). The results demonstrated that hPDLs under intermittent compressive stress exhibited significantly higher NOTCH2, NOTCH3, HES1, and HEY1 mRNA expression compared with control, indicating that mechanical stress induced Notch signaling. DAPT pretreatment markedly reduced the intermittent stress‐induced SOST expression. The expression of NOTCH2, NOTCH3, HES1, and HEY1 mRNA under compressive stress was significantly reduced after pretreatment with SB431542, coinciding with a reduction in SOST expression. Recombinant human TGF‐β1 enhanced SOST, Notch receptor, and target gene expression in hPDLs. Further, DAPT treatment attenuated rhTGF‐β1‐induced SOST expression. In summary, intermittent compressive stress regulates Notch receptor and target gene expression via the TGF‐β signaling pathway. In addition, Notch signaling participates in TGF‐β‐induced SOST expression in hPDLs. J. Cell. Physiol. 232: 2221–2230, 2017. © 2016 Wiley Periodicals, Inc. Intermittent stress stimulates TGF‐β signaling via an unknown mechanism. TGF‐β, in turn, induces the expression of SOST, NOTCH2, NOTCH3, HES1, and HEY1, whose expression was reduced by SB431542. Inhibition SOST expression, but not that of TGF‐β by DAPT and NOTCH2 shRNA indicate that NOTCH signaling is a downstream participant in the TGF‐β‐induced SOST expression.