Although various stakeholders are involved in public communication about science and technology, research so far has focused on scientists, journalists, and the public. Based on representative telephone surveys of the spokespersons of 55 German companies, 31 government agencies, 43 public interest groups, as well as 105 scientists, we investigated actors’ intentions to point out the scientific uncertainty of biotechnological research in their public communications. The different groups of actors’ intentions to mention uncertain aspects of biotechnological research in public are guided by different rationales. Scientists and company representatives’ intentions to point out uncertainty are strengthened by their hope to promote biotechnological research and weakened by their fear to increase public criticism. Public interest groups’ intentions are strengthened by their hope to increase public criticism and are weakened by their fear to promote biotechnological research. Representatives of government agencies are predominantly influenced by their will to do justice to their organizations’ interests.