Through the concept of entanglement, archaeological indications of cultural identity and skeletal evidence of biological and geographic interaction are used to explore the development of the Nubian polity who ruled as the 25th Dynasty of Egypt (Napatan period, ca. 750–656 B.C.E.). In this article, we examine the ways in which cultural and biological linkages affect the political, social, and cultural trajectories of the political entities in the ancient Nile Valley. Early studies of political developments in this region have often focused on Egypt, ignoring the aspects of power formation that may have developed independently and the long tradition of established local institutions in Nubia. The present research uses evidence from the site of Tombos, located in Upper Nubia, to investigate the processes of identity formation and population composition during the Egyptian colonial occupation and the subsequent rise of the Nubian Napatan polity. We address the impact of Egyptian and Nubian immigrants on the political developments, finding strongest support for the influence of Nubian‐Egyptian communities established in colonial times on the character of the Napatan polity. [cultural entanglement, Egypt, 25th Dynasty, Tombos, mortuary practices, state formation]