This study examined the career and learning motivation of students studying a Master of Library and Information Science (MLIS) degree programs at universities located in four different countries, namely the University of Hong Kong, National Taiwan Normal University, Peking University, and the University of Copenhagen. The MLIS students from these four universities were invited to take part in a self-completion online questionnaire survey, and 200 responses were collected in total. We discovered that their prior working experience in the library had an impact on their decision to pursue the MLIS program. We also categorized the career and learning motivation factors of MLIS learners into intrinsic and extrinsic factors, and discovered that the participants were motivated by both intrinsic and extrinsic factors to pursue their graduate studies in the Library and Information Science (LIS) field. We found that intrinsic factors influenced the students of University of Copenhagen significantly more than the other student groups, and this could be explained by Hofstede’s cultural dimensions. Our findings can assist with the formulation of development strategies for MLIS programs through a review of the LIS curriculum and help MLIS programs in different countries attract more people to pursue a career in LIS.