Matrix extension to bovine feces and evaluation of semi-automated DNA extraction methods for the detection of Campylobacter jejuni in bovine and canine fecal samples using gyrA PCR

Sara D. Lawhon, Jing Wu, Kristy L. Pabilonia, Zeinab Helal, Guillermo Risatti, Alex Nemethy, Jeremy N. Ray, David Simon, Wanda Tirado, Lijuan Zhou, Paula Bartlett, Ingrid Fernandez, Susan Sanchez, Vanessa DeShambo, Namjung Jung, Carol Maddox, Steven Ensley, Udeni Balasuriya, Keith Strother, Lifang Yan, Candy Zhang, Zhenyu Shen, Shuping Zhang, Sarmila Dasgupta, Shannon Mann, Amar Patil, Laura B. Goodman, Kelly Sams, Jing Cui, Melanie Prarat Koscielny, Qirui Zhang, Yan Zhang, Robin Madden, Akhilesh Ramachandran, Jessica Bower, Li Fang, Deepanker Tewari, Nagaraja Thirumalapura, Corey Zellers, Jake Guag, Sarah M. Nemser, Gregory H. Tyson

Journal of Veterinary Diagnostic Investigation

Published online on July 03, 2026

Abstract

Journal of Veterinary Diagnostic Investigation, Ahead of Print.
We evaluated a semi-automated method for DNA extraction, expanded to herbivore feces agyrAPCR method for the detection ofCampylobacter jejuniin canine feces, added an internal positive control (IPC) DNA to specimens before DNA extraction, and ...