Intermediate crack-induced debonding is often a dominant failure mode in fiber-reinforced polymer (FRP)-strengthened reinforced concrete (RC) beams in flexure. It has been extensively studied for RC beams externally strengthened with unstressed FRP laminates. However, very little work has been done on FRP debonding for RC beams strengthened with prestressed FRP. This article presents a sectional analysis model for predicting the flexural capacity of RC beams strengthened with prestressed FRP laminates with due consideration of different failure modes. The focus is placed on the effective strain in the prestressed FRP at the ultimate states of intermediate crack-induced debonding or rupture of debonded FRP. Through back-calculation analysis of 51 RC beams strengthened with post-tensioned FRP laminates, a model for predicting the effective strain of prestressed FRP for ultimate strength prediction based upon sectional analyses was developed and validated through comparisons with test results.